Abstract:

 Objective To explore the correlation between age and β-amyloid protein (Aβ) content of aqueous humor in patients undergoing cataract surgery at different ages. Design Experimental study. Participants 210 patients with cataract treated by surgery, which mean age was 72.33±10.35 years old. Method All participants underwent systematic and ophthalmic examinations. When cataract surgery, the sample of aqueous humor was extracted with anterior chamber paracentesis. The contents of Aβ1-40, Aβ1-42 were detected with ELISA method. All the subjects were divided by age: group A (< 60 years), group B (60-69 years old), group C (60-79 years), group D (≥80 years). The differences of contents of Aβ1-40, Aβ1-42 in aqueous humor were compared among different age groups, the relationship between the contents of Aβ1-40, Aβ1-42 in aqueous humor and age was investigated. Main Outcome Measures The contents of Aβ1-40 and Aβ1-42 in aqueous humor. Results The content of Aβ1-40 in aqueous humor in group A, B, C, D was 80.91±9.33, 85.81±8.97, 85.46±11.22, 82.25±10.41 ng/ml respectively (F=2.399, P=0.069). The content of Aβ1-42 in aqueous humor in group A, B, C, D was 41.98±7.26, 42.46±5.75, 43.57±5.27, 42.80±6.04 ng/ml (F=0.719, P=0.543). Aβ1-40/Aβ1-42 ratio in group A, B, C, D was 1.97±0.34, 2.04±0.23, 1.98±0.27, 1.95±0.33 (F=0.680, P=0.565). Conclusions There were no significant difference of Aβ1-40, Aβ1-42 and Aβ1-40/Aβ1-42 ratio in aqueous humor of patients with cataract at different ages. The contents of Aβ-amyloid protein in aqueous humor have no obvious change with age. If the Aβ-amyloid protein content in the aqueous humor changes markedly, there may be a disease associated with impaired amyloid metabolism. (Ophthalmol CHN, 2017, 26: 282-285)

Key words: aqueous humor, β-amyloidprotein